Drosophila histone locus body assembly and function involves multiple interactions.

ABSTRACT: The histone locus body (HLB) assembles at replication-dependent (RD) histone loci and concentrates factors required for RD histone mRNA biosynthesis. The Drosophila melanogaster genome has a single locus comprised of ∼100 copies of a tandemly arrayed 5-kB repeat unit containing one copy of each of the 5 RD histone genes. To determine sequence elements required for D. melanogaster HLB formation and histone gene expression, we used transgenic gene arrays containing 12 copies of the histone repeat unit that functionally complement loss of the ∼200 endogenous RD histone genes. A 12x histone gene array in which all H3-H4 promoters were replaced with H2a-H2b promoters (12x^PR) does not form an HLB or express high levels of RD histone mRNA in the presence of

SUBMITTER: Koreski KP 

PROVIDER: S-EPMC7359574 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature