In vivo creation of plasmid pCRT01 and its use for the construction of carotenoid-producing Paracoccus spp. strains that grow efficiently on industrial wastes.

ABSTRACT:

Background

Carotenoids are natural tetraterpene pigments widely utilized in the food, pharmaceutical and cosmetic industries. Currently, chemical synthesis of these compounds outperforms their production in Escherichia coli or yeast due to the limited efficiency of the latter. The use of natural microbial carotenoid producers, such as bacteria of the genus Paracoccus (Alphaproteobacteria), may help to optimize this process. In order to couple the ability to synthesize these pigments with the metabolic versatility of this genus, we explored the possibility of introducing carotenoid synthesis genes into strains capable of efficient growth on simple low-cost media.

Results

We constructed two carotenoid-producing strains of Paracoccus carrying a new plasmid, pCRT01, which contai

SUBMITTER: Maj A 

PROVIDER: S-EPMC7359593 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature