An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length.

ABSTRACT: Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are essential for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and a range of disease states. Different methods such as terminal restriction fragment analysis, real-time quantitative PCR (qPCR) and fluorescence in situ hybridization are available to measure telomere length; however, the qPCR-based method is commonly used for large population-based studies. There are multiple variations across qPCR-based methods, including the choice of the single-copy gene, primer sequences, reagents, and data analysis methods in the different reported studies so far. Here, we

SUBMITTER: Joglekar MV 

PROVIDER: S-EPMC7359711 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature