Detection limit of ⁸⁹Zr-labeled T cells for cellular tracking: an in vitro imaging approach using clinical PET/CT and PET/MRI.

ABSTRACT:

Purpose

Tracking cells in vivo using imaging can provide non-invasive information to understand the pharmacology, efficacy, and safety of novel cell therapies. Zirconium-89 (t_1/2 = 78.4 h) has recently been used to synthesize [⁸⁹Zr]Zr(oxinate)₄ for cell tracking using positron emission tomography (PET). This work presents an in vitro approach to estimate the detection limit for in vivo PET imaging of Jurkat T cells directly labeled with [⁸⁹Zr]Zr(oxinate)₄ utilizing clinical PET/CT and PET/MRI.

Methods

Jurkat T cells were labeled with varying concentrations of [⁸⁹Zr]Zr(oxinate)₄ to generate different cell-specific activities (0.43-31.91 kBq/10⁶ cells). Different concentrations of labeled c

SUBMITTER: Lechermann LM 

PROVIDER: S-EPMC7360010 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature