N⁶-Methyladenosine Modulates Nonsense-Mediated mRNA Decay in Human Glioblastoma.

ABSTRACT: The N⁶-methyladenosine (m⁶A) modification influences various mRNA metabolic events and tumorigenesis, however, its functions in nonsense-mediated mRNA decay (NMD) and whether NMD detects induced carcinogenesis pathways remain undefined. Here, we showed that the m⁶A methyltransferase METTL3 sustained its oncogenic role by modulating NMD of splicing factors and alternative splicing isoform switches in glioblastoma (GBM). Methylated RNA immunoprecipitation-seq (MeRIP-seq) analyses showed that m⁶A modification peaks were enriched at metabolic pathway-related transcripts in glioma stem cells (GSC) compared with neural progenitor cells. In addition, the clinical aggressiveness of malignant gliomas was associated with elevated expression of METTL3. Furt

SUBMITTER: Li F 

PROVIDER: S-EPMC7360104 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature