Electrode-Free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations.

ABSTRACT: Advances in microanalytical and microfluidic technologies have enabled rapid and amplification-free detection of DNA with a high signal-to-noise ratio. The low sample volume, however, poses a limit in the DNA detection sensitivity, which can be challenging for analyzing rare DNA in physiological samples. One way to improve the sensitivity is to concentrate the DNA in the sample prior to the analysis. The most common DNA concentration techniques are based on electrokinetics, which require an external electric field and generally become ineffective in high ionic concentration conditions. In this work, we present a facile method termed high-salt molecular rheotaxis (HiSMRT) to concentrate and recover DNA from samples with physiologically relevant ionic concentrations without any external elec

SUBMITTER: Li S 

PROVIDER: S-EPMC7360426 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature