Overcoming polyploidy pitfalls: a user guide for effective SNP conversion into KASP markers in wheat.

ABSTRACT:

Key message

Conversion of SNP chip assays into locus-specific KASP markers requires adapted strategies in polyploid species with high genome homeology. Procedures are exemplified by QTL-associated SNPs in hexaploid wheat. Kompetitive allele-specific PCR (KASP) markers are commonly used in marker-assisted commercial plant breeding due to their cost-effectiveness and throughput for high sample volumes. However, conversion of trait-linked SNP markers from array-based SNP detection technologies into KASP markers is particularly challenging in polyploid crop species, due to the presence of highly similar homeologous and paralogous genome sequences. We evaluated strategies and identified key requirements for successful conversion of Illumina Infinium assays from the wheat 90 K SNP array

SUBMITTER: Makhoul M 

PROVIDER: S-EPMC7360542 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature