Uranium-free X solution: a new generation contrast agent for biological samples ultrastructure.

ABSTRACT: Biological samples are mainly composed of elements with a low atomic number which show a relatively low electron scattering power. For Transmission Electron Microscopy analysis, biological samples are generally embedded in resins, which allow thin sectioning of the specimen. Embedding resins are also composed by light atoms, thus the contrast difference between the biological sample and the surrounding resin is minimal. Due to that reason in the last decades, several staining solutions and approaches, performed with heavy metal salts, have been developed with the purpose of enhancing both the intrinsic sample contrast and the differences between the sample and resin. The best staining was achieved with the uranyl acetate (UA) solution, which has been the election method for the study of mo

SUBMITTER: Moscardini A 

PROVIDER: S-EPMC7360580 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature