Characterization of multicellular breast tumor spheroids using image data-driven biophysical mathematical modeling.

ABSTRACT: Multicellular tumor spheroid (MCTS) systems provide an in vitro cell culture model system which mimics many of the complexities of an in vivo solid tumor and tumor microenvironment, and are often used to study cancer cell growth and drug efficacy. Here, we present a coupled experimental-computational framework to estimate phenotypic growth and biophysical tumor microenvironment properties. This novel framework utilizes standard microscopy imaging of MCTS systems to drive a biophysical mathematical model of MCTS growth and mechanical interactions. By extending our previous in vivo mechanically-coupled reaction-diffusion modeling framework we developed a microscopy image processing framework capable of mechanistic characterization of MCTS systems. Using MDA-MB-231 breast cancer MCTS, we esti

SUBMITTER: Bowers HJ 

PROVIDER: S-EPMC7360601 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature