Glycogen accumulation, central carbon metabolism, and aging of hematopoietic stem and progenitor cells.

ABSTRACT: Inspired by recent proteomic data demonstrating the upregulation of carbon and glycogen metabolism in aging human hematopoietic stem and progenitor cells (HPCs, CD34+ cells), this report addresses whether this is caused by elevated glycolysis of the HPCs on a per cell basis, or by a subpopulation that has become more glycolytic. The average glycogen content in individual CD34+ cells from older subjects (> 50 years) was 3.5 times higher and more heterogeneous compared to younger subjects (< 35 years). Representative glycolytic enzyme activities in HPCs confirmed a significant increase in glycolysis in older subjects. The HPCs from older subjects can be fractionated into three distinct subsets with high, intermediate, and low glucose uptake (GU) capacity, while the subset with a high GU capa

SUBMITTER: Poisa-Beiro L 

PROVIDER: S-EPMC7360735 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature