Four-step approach to efficiently develop capillary gel electrophoresis methods for viral vaccine protein analysis.

ABSTRACT: Vaccines against infectious diseases are urgently needed. Therefore, modern analytical method development should be as efficient as possible to speed up vaccine development. The objectives of the study were to identify critical method parameters (CMPs) and to establish a set of steps to efficiently develop and validate a CE-SDS method for vaccine protein analysis based on a commercially available gel buffer. The CMPs were obtained from reviewing the literature and testing the effects of gel buffer dilution. A four-step approach, including two multivariate DoE (design of experiments) steps, was proposed, based on CMPs and was verified by CE-SDS method development for: (i) the determination of influenza group 1 mini-hemagglutinin glycoprotein; and (ii) the determination of polio virus partic

SUBMITTER: Geurink L 

PROVIDER: S-EPMC7361255 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature