Collision-Induced Dissociation Studies of Protonated Ions of Alkylated Thymidine and 2'-Deoxyguanosine.

ABSTRACT: Mass spectrometry and tandem MS (MS/MS) have been widely employed for the identification and quantification of damaged nucleosides in DNA, including those induced by alkylating agents. Upon collisional activation, protonated ions of alkylated nucleosides frequently undergo facile neutral loss of a 2-deoxyribose in MS/MS, and further cleavage of the resulting protonated nucleobases in MS³ can sometimes be employed for differentiating regioisomeric alkylated DNA lesions. Herein, we investigated systematically the collision-induced dissociation (CID) of the protonated ions of O⁴-alkylthymidine (O⁴-alkyldT), O²-alkyldT, O⁶-alkyl-2'-deoxyguanosine (O⁶-alkyldG), and N²-alkyldG t

SUBMITTER: Cui Y 

PROVIDER: S-EPMC7362892 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature