Approaches to High-Throughput Analysis of Cardiomyocyte Contractility.

ABSTRACT: The measurement of the contractile behavior of single cardiomyocytes has made a significant contribution to our understanding of the physiology and pathophysiology of the myocardium. However, the isolation of cardiomyocytes introduces various technical and statistical issues. Traditional video and fluorescence microscopy techniques based around conventional microscopy systems result in low-throughput experimental studies, in which single cells are studied over the course of a pharmacological or physiological intervention. We describe a new approach to these experiments made possible with a new piece of instrumentation, the CytoCypher High-Throughput System (CC-HTS). We can assess the shortening of sarcomeres, cell length, Ca²⁺ handling, and cellular morphology of almost 4 cells

SUBMITTER: Wright PT 

PROVIDER: S-EPMC7362994 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature