Automated quantification of head-twitch response in mice via ear tag reporter coupled with biphasic detection.

ABSTRACT:

Background

Head-twitch response (HTR) is a manifestation of the serotonergic system behavioral pharmacology commonly used as a proxy of psychedelic drug action in rodents.

New method

We developed a minimally invasive magnetic ear tag reporter and designed a detection system that performs a comprehensive characterization of each potential HTR event on an electromagnetic readout.

Results

Magnetic ear tags were easy to install and generally well tolerated by the animals. On the low-threshold first phase of detection, the tags' signal recorded in a magnetometer was filtered and screened for potential HTR events. On the second phase, the detector performed a comprehensive spectral analysis evaluation of each event and identified the HTR characteristic distribution of power

SUBMITTER: de la Fuente Revenga M 

PROVIDER: S-EPMC7363508 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature