Dataset for reproducing absorption spectra of methyl orange from the RGB values of microscopic images.

ABSTRACT: The present dataset is related to the research paper entitled "Reproducing Absorption Spectra of pH Indicators from RGB Values of Microscopic Images" (Inagawa et al., 2020). The dataset contains microscopic images of aqueous methyl orange (MO), absorption spectra acquired with a spectrophotometer, loading spectra and calculation sheets for reproducing absorption spectra of aqueous MO from their RGB values of the microscopic images. The microscopic transmission images of the standard MO solutions at various pH conditions were acquired with a CMOS camera equipped with an invert microscope. Meanwhile, the loading spectra were obtained by principle component analysis of a series of absorption spectra of the standard solutions. The conversion matrix from RGB values in a region of interests (ROI

SUBMITTER: Inagawa A 

PROVIDER: S-EPMC7363659 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature