Expression and function of an Hac1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae-based expression systems, which rely on safe, food-grade strains, are low cost, simple to operate, and can be used for large-scale fermentation. However, low levels of foreign protein expression by S. cerevisiae have limited their widespread application. The ability of the endoplasmic reticulum (ER) to fold and process foreign proteins is an important factor restricting the expression of foreign proteins. In the current study, the effects of transcription factor Hac1p, which is involved in the unfolded protein response pathway, on S. cerevisiae-based expression of xylanase gene xynB from Aspergillus niger were examined. Overlap extension polymerase chain reaction (PCR), rDNA integration and droplet digital PCR technology were used to generate a S. cerevisiae strain

SUBMITTER: Bao C 

PROVIDER: S-EPMC7363925 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature