MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications.

ABSTRACT: Epigenetic mechanisms such as histone modifications play critical roles in adaptive tuning of chromatin structures. Profiling of various histone modifications at the genome scale using tissues from animal and human samples is an important step for functional studies of epigenomes and epigenomics-based precision medicine. Because the profile of a histone mark is highly specific to a cell type, cell isolation from tissues is often necessary to generate a homogeneous cell population, and such operations tend to yield a low number of cells. In addition, high-throughput processing is often desirable because of the multiplexity of histone marks of interest and the large quantity of samples in a hospital setting. In this protocol, we provide detailed instructions for device fabrication, setup, an

SUBMITTER: Zhu B 

PROVIDER: S-EPMC7364495 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature