Genome Scale Metabolic Model of the versatile methanotroph Methylocella silvestris.

ABSTRACT:

Background

Methylocella silvestris is a facultative aerobic methanotrophic bacterium which uses not only methane, but also other alkanes such as ethane and propane, as carbon and energy sources. Its high metabolic versatility, together with the availability of tools for its genetic engineering, make it a very promising platform for metabolic engineering and industrial biotechnology using natural gas as substrate.

Results

The first Genome Scale Metabolic Model for M. silvestris is presented. The model has been used to predict the ability of M. silvestris to grow on 12 different substrates, the growth phenotype of two deletion mutants (ΔICL and ΔMS), and biomass yield on methane and ethanol. The model, together with phenotypic characterization of the deletion mutants, revealed

SUBMITTER: Bordel S 

PROVIDER: S-EPMC7364539 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature