ADAM15 expression is increased in lung CD8⁺ T cells, macrophages, and bronchial epithelial cells in patients with COPD and is inversely related to airflow obstruction.

ABSTRACT:

Background

A disintegrin and metalloproteinase domain-15 (ADAM15) is expressed by activated leukocytes, and fibroblasts in vitro. Whether ADAM15 expression is increased in the lungs of COPD patients is not known.

Methods

ADAM15 gene expression and/or protein levels were measured in whole lung and bronchoalveolar lavage (BAL) macrophage samples obtained from COPD patients, smokers, and non-smokers. Soluble ADAM15 protein levels were measured in BAL fluid (BALF) and plasma samples from COPD patients and controls. Cells expressing ADAM15 in the lungs were identified using immunostaining. Staining for ADAM15 in different cells in the lungs was related to forced expiratory volume in 1 s (FEV₁), ratio of FEV₁ to forced vital capacity (FEV₁/FVC), an

SUBMITTER: Wang X 

PROVIDER: S-EPMC7364636 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature