Urban airborne PM_2.5-activated microglia mediate neurotoxicity through glutaminase-containing extracellular vesicles in olfactory bulb.

ABSTRACT: Emerging evidence has showed that exposure to airborne particulate matter (PM) with an aerodynamic diameter less than 2.5 μm (PM_2.5) is associated with neurodegeneration. Our previous studies in vitro found that PM_2.5 exposure causes primary neurons damage through activating microglia. However, the molecular mechanism of microglia-mediated neurotoxicity remains to elucidate. In this study, five groups (N = 13 or 10) of six-week-old male C57BL/6 mice were daily exposed to PM_2.5 (0.1 or 1 mg/kg/day body weight), Chelex-treated PM_2.5 (1 mg/kg/day body weight), PM_2.5 (1 mg/kg/day body weight) plus CB-839 (glutaminase inhibitor), or deionized water by intranasal instillation for 28 days, respectively. Compared with the control groups, We fo

SUBMITTER: Chen X 

PROVIDER: S-EPMC7364855 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature