Assaying Circuit Specific Regulation of Adult Hippocampal Neural Precursor Cells.

ABSTRACT: Adult neurogenesis is a dynamic process by which newly activated neural stem cells (NSCs) in the subgranular zone (SGZ) of the dentate gyrus (DG) generate new neurons, which integrate into an existing neural circuit and contribute to specific hippocampal functions. Importantly, adult neurogenesis is highly susceptible to environmental stimuli, which allows for activity-dependent regulation of various cognitive functions. A vast range of neural circuits from various brain regions orchestrates these complex cognitive functions. It is therefore important to understand how specific neural circuits regulate adult neurogenesis. Here, we describe a protocol to manipulate neural circuit activity using designer receptor exclusively activated by designer drugs (DREADDs) technology that regulates NSC

SUBMITTER: Quintanilla LJ 

PROVIDER: S-EPMC7365713 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature