Sliced Human Cortical Organoids for Modeling Distinct Cortical Layer Formation.

ABSTRACT: Human brain organoids provide unique platforms for modeling development and diseases by recapitulating the architecture of the embryonic brain. However, current organoid methods are limited by interior hypoxia and cell death due to insufficient surface diffusion, preventing generation of architecture resembling late developmental stages. Here, we report the sliced neocortical organoid (SNO) system, which bypasses the diffusion limit to prevent cell death over long-term cultures. This method leads to sustained neurogenesis and formation of an expanded cortical plate that establishes distinct upper and deep cortical layers for neurons and astrocytes, resembling the third trimester embryonic human neocortex. Using the SNO system, we further identify a critical role of WNT/β-catenin signaling

SUBMITTER: Qian X 

PROVIDER: S-EPMC7366517 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature