An ex vivo Approach to Study Hormonal Control of Spermatogenesis in the Teleost Oreochromis niloticus.

ABSTRACT: As the male reproductive organ, the main task of the testis is the production of fertile, haploid spermatozoa. This process, named spermatogenesis, starts with spermatogonial stem cells, which undergo a species-specific number of mitotic divisions until starting meiosis and further morphological maturation. The pituitary gonadotropins, luteinizing hormone, and follicle stimulating hormone, are indispensable for vertebrate spermatogenesis, but we are still far from fully understanding the complex regulatory networks involved in this process. Therefore, we developed an ex vivo testis cultivation system which allows evaluating the occurring changes in histology and gene expression. The experimental circulatory flow-through setup described in this work provides the possibility to study

SUBMITTER: Thonnes M 

PROVIDER: S-EPMC7366826 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature