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Allostery of DNA nanostructures controlled by enzymatic modifications.

ABSTRACT: Allostery is comprehensively studied for natural macromolecules, such as proteins and nucleic acids. Here, we present controllable allostery of synthetic DNA nanostructure-enzyme systems. Rational designs of the synthetic allosteric systems are based on an in-depth understanding of allosteric sites with several types of strand placements, whose varying stacking strengths determine the local conformation and ultimately lead to a gradient level of allosteric transition. When enzymes in a molecular cloning toolbox such as DNA polymerase, exonuclease and ligase are applied to treat the allosteric sites, the resulting local conformational changes propagate through the entire structure for a global allosteric transition.

SUBMITTER: Yan Q

PROVIDER: S-EPMC7367186 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Allostery of DNA nanostructures controlled by enzymatic modifications.

Yan Qi Q Wang Yaqi Y Shi Jile J Wei Bryan B

Nucleic acids research 20200701 13

Allostery is comprehensively studied for natural macromolecules, such as proteins and nucleic acids. Here, we present controllable allostery of synthetic DNA nanostructure-enzyme systems. Rational designs of the synthetic allosteric systems are based on an in-depth understanding of allosteric sites with several types of strand placements, whose varying stacking strengths determine the local conformation and ultimately lead to a gradient level of allosteric transition. When enzymes in a molecular ...[more]

PMID: 32526030

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