Precise annotation of tick mitochondrial genomes reveals multiple copy number variation of short tandem repeats and one transposon-like element.

ABSTRACT:

Background

In the present study, we used long-PCR amplification coupled with Next-Generation Sequencing (NGS) to obtain complete mitochondrial (mt) genomes of individual ticks and unprecedently performed precise annotation of these mt genomes. We aimed to: (1) develop a simple, cost-effective and accurate method for the study of extremely high AT-content mt genomes within an individual animal (e.g. Dermacentor silvarum) containing miniscule DNA; (2) provide a high-quality reference genome for D. silvarum with precise annotation and also for future studies of other tick mt genomes; and (3) detect and analyze mt DNA variation within an individual tick.

Results

These annotations were confirmed by the PacBio full-length transcriptome data to cover both entire strands of the mito

SUBMITTER: Chen Z 

PROVIDER: S-EPMC7367389 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature