Regulatory circuits controlling Spx levels in Streptococcus mutans.

ABSTRACT: Spx is a major regulator of stress responses in Firmicutes. In Streptococcus mutans, two Spx homologues, SpxA1 and SpxA2, were identified as mediators of oxidative stress responses but the regulatory circuits controlling their levels and activity are presently unknown. Comparison of SpxA1 and SpxA2 protein sequences revealed differences at the C-terminal end, with SpxA1 containing an unusual number of acidic residues. Here, we showed that a green fluorescence protein (GFP) reporter becomes unstable when fused to the last 10 amino acids of SpxA2 but remained stable when fused to the C-terminal acidic tail of SpxA1. Inactivation of clpP or simultaneous inactivation of clpC and clpE stabilized the GFP::SpxA2_tail fusion protein. Addition of acidic amino acids to the GFP::SpxA2_t

SUBMITTER: Ganguly T 

PROVIDER: S-EPMC7367440 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature