LncRNA TMPO-AS1 promotes hepatocellular carcinoma cell proliferation, migration and invasion through sponging miR-329-3p to stimulate FOXK1-mediated AKT/mTOR signaling pathway.

ABSTRACT:

Purpose

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. Numerous analyses have revealed the abnormal expression of long non-coding RNAs (lncRNAs) in HCC cells. This study aims to explore biological functions of lncRNA TMPO-AS1 (TMPO antisense RNA 1) in HCC cell proliferation, apoptosis, invasion and migration.

Methods

The gene expression in HCC tissues and cell lines were measured by qRT-PCR. The role of TMPO-AS1 in HCC was confirmed by CCK-8, colony formation, TUNEL, transwell and western blot as well as by in vivo experiments. RNA pull down and luciferase reporter assays were utilized to prove the binding relationship between TMPO-AS1/FOXK1 (forkhead box K1) andmiR-329-3p. Rescue assays elucidated the regulatory effects of TMP

SUBMITTER: Guo X 

PROVIDER: S-EPMC7367632 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature