Wee1 Kinase Inhibitor AZD1775 Effectively Sensitizes Esophageal Cancer to Radiotherapy.

ABSTRACT:

Purpose

Esophageal cancer is a deadly malignancy with a 5-year survival rate of only 5% to 20%, which has remained unchanged for decades. Esophageal cancer possesses a high frequency of TP53 mutations leading to dysfunctional G₁ cell-cycle checkpoint, which likely makes esophageal cancer cells highly reliant upon G₂-M checkpoint for adaptation to DNA replication stress and DNA damage after radiation. We aim to explore whether targeting Wee1 kinase to abolish G₂-M checkpoint sensitizes esophageal cancer cells to radiotherapy.

Experimental design

Cell viability was assessed by cytotoxicity and colony-forming assays, cell-cycle distribution was analyzed by flow cytometry, and mitotic catastrophe was assessed by immunofluorescence staining

SUBMITTER: Yang L 

PROVIDER: S-EPMC7367716 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature