A universal and independent synthetic DNA ladder for the quantitative measurement of genomic features.

ABSTRACT: Standard units of measurement are required for the quantitative description of nature; however, few standard units have been established for genomics to date. Here, we have developed a synthetic DNA ladder that defines a quantitative standard unit that can measure DNA sequence abundance within a next-generation sequencing library. The ladder can be spiked into a DNA sample, and act as an internal scale that measures quantitative genetics features. Unlike previous spike-ins, the ladder is encoded within a single molecule, and can be equivalently and independently synthesized by different laboratories. We show how the ladder can measure diverse quantitative features, including human genetic variation and microbial abundance, and also estimate uncertainty due to technical variation and improv

SUBMITTER: Reis ALM 

PROVIDER: S-EPMC7367866 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature