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Cofactor-enabled functional expression of fruit fly, honeybee, and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions.

ABSTRACT: The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in Xenopus laevis oocytes of honeybee (Apis mellifera) and bumblebee (Bombus terrestris) as well as fruit fly (Drosophila melanogaster) nAChR heteromers targeted by neonicotinoids and not hitherto robustly expressed. This has enabled the characterization of picomolar target site actions of neonicotinoids, findings important in understanding their toxicity.

SUBMITTER: Ihara M 

PROVIDER: S-EPMC7368294 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Cofactor-enabled functional expression of fruit fly, honeybee, and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions.

Ihara Makoto M   Furutani Shogo S   Shigetou Sho S   Shimada Shota S   Niki Kunihiro K   Komori Yuma Y   Kamiya Masaki M   Koizumi Wataru W   Magara Leo L   Hikida Mai M   Noguchi Akira A   Okuhara Daiki D   Yoshinari Yuto Y   Kondo Shu S   Tanimoto Hiromu H   Niwa Ryusuke R   Sattelle David B DB   Matsuda Kazuhiko K  

Proceedings of the National Academy of Sciences of the United States of America 20200701 28

The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in <i>Xenopus laevis</i> oocytes of honeybee (<i>Apis mellifera</i>) a  ...[more]

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