Biofilm released cells can easily be obtained in a fed-batch system using ica+ but not with ica- isolates.

ABSTRACT: Staphylococcus epidermidis is one of the major opportunistic bacterial pathogens in healthcare facilities, mainly due to its strong ability to form biofilms in the surface of indwelling medical devices. To study biofilms under in vitro conditions, both fed-batch and flow systems are widely used, with the first being the most frequent due to their low cost and ease of use.

Aim

To assess if a fed-batch system previously developed to obtain biofilm released cells (Brc) from strong biofilm producing S. epidermidis isolates could also be used to obtain and characterize Brc from isolates with lower abilities to form biofilms.

Methodology

The applicability of a fed-batch system to obtain Brc from biofilms of 3 ica ⁺ and 3 ica ^- isola

SUBMITTER: Gaio V 

PROVIDER: S-EPMC7368429 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature