An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility.

ABSTRACT: With immunohistochemical (IHC) staining increasingly being used to guide clinical decisions, variability in staining quality and reproducibility are becoming essential factors in generating diagnoses using IHC tissue preparations. The current study tested a method to track and quantify the interrun, intrarun, and intersite variability of IHC staining intensity. Our hypothesis was that staining precision between laboratory sites, staining runs, and individual slides may be verified quantitatively, efficiently and effectively utilizing algorithm-based, automated image analysis. To investigate this premise, we tested the consistency of IHC staining in 40 routinely processed (formalin-fixed, paraffin-embedded) human tissues using 10 common antibiomarker antibodies on 2 Dako Omnis instruments a

SUBMITTER: Chlipala EA 

PROVIDER: S-EPMC7368846 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature