Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization.

ABSTRACT: Generating robust CD4⁺ T-helper cell type 1 (Th1) responses is essential for protective vaccine-induced type 1 immunity. Here, we examine whether immunization formulation associated with enhanced vaccine efficacy promotes antigen targeting and cell recruitment into lymph node (LN) niches associated with optimal type 1 responses. Immunization with antigen and Toll-like receptor agonist emulsified in oil leads to an increased differentiation of IFNγ/TNF-α⁺ polyfunctional Th1 cells compared to an identical immunization in saline. Oil immunization results in a rapid delivery and persistence of antigen in interfollicular regions (IFRs) of the LN, whereas without oil, antigen is distributed in the medullary region. Following oil immunization, CXCL10-producing inflammatory m

SUBMITTER: Lian J 

PROVIDER: S-EPMC7369031 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature