A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition.

ABSTRACT: The three-dimensional structures of chromosomes are increasingly being recognized as playing a major role in cellular regulatory states. The efficiency and promiscuity of phage Mu transposition was exploited to directly measure in vivo interactions between genomic loci in E. coli. Two global organizing principles have emerged: first, the chromosome is well-mixed and uncompartmentalized, with transpositions occurring freely between all measured loci; second, several gene families/regions show "clustering": strong three-dimensional co-localization regardless of linear genomic distance. The activities of the SMC/condensin protein MukB and nucleoid-compacting protein subunit HU-α are essential for the well-mixed state; HU-α is also needed for clustering of 6/7 ribosomal RNA-encoding loci. The

SUBMITTER: Walker DM 

PROVIDER: S-EPMC7369145 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature