Imaging neuronal activity in the central and peripheral nervous systems using new Thy1.2-GCaMP6 transgenic mouse lines.

ABSTRACT:

Background

The genetically encoded calcium (Ca²⁺) sensor GCaMP6 has been widely used for imaging Ca²⁺ transients in neuronal somata, dendrites, and synapses.

New method

Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of theThy1.2 promoter.

Results

These transgenic lines exhibit stable and layer-specific expression of GCaMP6 in multiple brain regions. They have several unique features compared to existing Thy1.2-GCaMP6 mice, including sparse expression of GCaMP6 in layer V pyramidal neurons of the cerebral cortex, motor neurons in the spinal cord, as well as sensory neurons in dorsal root ganglia (DRG). We further demonstrate that these mous

SUBMITTER: Cichon J 

PROVIDER: S-EPMC7369242 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature