Exploring protein phosphorylation by combining computational approaches and biochemical methods.

ABSTRACT: Post-translational modifications of proteins expand their functional diversity, regulating the response of cells to a variety of stimuli. Among these modifications, phosphorylation is the most ubiquitous and plays a prominent role in cell signaling. The addition of a phosphate often affects the function of a protein by altering its structure and dynamics. However, these alterations are often difficult to study and the functional and structural implications remain unresolved. New approaches are emerging to overcome common obstacles related to the production and manipulation of these samples. Here, we summarize the available methods for phosphoprotein purification and phosphomimetic engineering, highlighting the advantages and disadvantages of each. We propose a general workflow for protein

SUBMITTER: Perez-Mejias G 

PROVIDER: S-EPMC7369424 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature