G6PC2 confers protection against hypoglycemia upon ketogenic diet feeding and prolonged fasting.

ABSTRACT:

Objective

G6PC2 is predominantly expressed in pancreatic islet beta cells. G6PC2 hydrolyzes glucose-6-phosphate to glucose and inorganic phosphate, thereby creating a futile substrate cycle that opposes the action of glucokinase. This substrate cycle determines the sensitivity of glucose-stimulated insulin secretion to glucose and hence regulates fasting blood glucose (FBG) but not fasting plasma insulin (FPI) levels. Our objective was to explore the physiological benefit this cycle confers.

Methods

We investigated the response of wild type (WT) and G6pc2 knockout (KO) mice to changes in nutrition.

Results

Pancreatic G6pc2 expression was little changed by ketogenic diet feeding but was inhibited by 24 hr fasting and strongly induced by high fat feeding. When challenge

SUBMITTER: Bosma KJ 

PROVIDER: S-EPMC7369601 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature