A DNA-Based FLIM Reporter for Simultaneous Quantification of Lysosomal pH and Ca²⁺ during Autophagy Regulation.

ABSTRACT: pH and Ca²⁺ play important roles in regulating lysosomal activity and lysosome-mediated physiological and pathological processes. However, effective methods for simultaneous determination of pH and Ca²⁺ is the bottleneck. Herein, a single DNA-based FLIM reporter was developed for real-time imaging and simultaneous quantification of pH and Ca²⁺ in lysosomes with high affinity, in which a specific probe for recognition of Ca²⁺ was assembled onto a DNA nanostructure together with pH-responsive and lysosome-targeted molecules. The developed DNA reporter showed excellent biocompatibility and long-term stability up to ∼56 h in lysosomes. Using this powerful tool, it was discovered that pH was closely related to Ca²⁺ concentration in lysosome

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC7369617 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature