An inhalation anaesthesia approach for neonatal mice allowing streamlined stereotactic injection in the brain.

ABSTRACT:

Background

Investigating brain function requires tools and techniques to visualise, modify and manipulate neuronal tissue. One powerful and popular method is intracerebral injection of customised viruses, allowing expression of exogenous transgenes. This technique is a standard procedure for adult mice, and is used by laboratories worldwide. Use of neonatal animals in scientific research allows investigation of developing tissues and enables long-term study of cell populations. However, procedures on neonatal mice are more challenging, due to the lack of reliable methods and apparatus for anaesthesia of these animals.

New method

Here, we report an inhalation-based protocol for anaesthesia of neonatal (P0-2) mice and present a custom 3D-printed apparatus for maintenance of an

SUBMITTER: Ho H 

PROVIDER: S-EPMC7369625 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature