Molecular Mapping of Hydrogen Sulfide Targets in Normal Human Keratinocytes.

ABSTRACT: Although sulfur-rich thermal waters have ancestrally been used in the context of dermatological conditions, a global mapping of the molecular effects exerted by H₂S on human keratinocytes is still lacking. To fill this knowledge gap, we subjected cultured human keratinocytes to distinct amounts of the non-gaseous hydrogen sulfur donor NaHS. We first checked that H₂S accumulated in the cytoplasm of keratinocytes under our experimental conditions andused a combination of proteomics, genomics and biochemical approaches to unravel functionally relevant H₂S targets in human keratinocytes. We found that the identified targets fall into two main categories: (i) the oxidative stress response molecules superoxide dismutase 2 (SOD2), NAD(P)H quinone dehydrogenase 1 (

SUBMITTER: Gross-Amat O 

PROVIDER: S-EPMC7369889 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature