PchE Regulation of Escherichia coli O157:H7 Flagella, Controlling the Transition to Host Cell Attachment.

ABSTRACT: Shiga toxins and intimate adhesion controlled by the locus of enterocyte effacement are major enterohemorrhagic Escherichia coli (EHEC) virulence factors. Curli fimbriae also contribute to cell adhesion and are essential biofilm components. The transcriptional regulator PchE represses the expression of curli and their adhesion to HEp-2 cells. Past studies indicate that pchE also represses additional adhesins that contribute to HEp-2 cell attachment. In this study, we tested for pchE regulation of several tissue adhesins and their regulators. Three adhesin-encoding genes (eae, lpfA1, fliC) and four master regulators (csgD, stpA, ler, flhDC) were controlled by pchE. pchE over-expression strongly up-regulated

SUBMITTER: Andreozzi E 

PROVIDER: S-EPMC7369912 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature