A Mitochondrial Micropeptide Is Required for Activation of the Nlrp3 Inflammasome.

ABSTRACT: Functional peptides encoded by short open reading frames are emerging as important mediators of fundamental biological processes. In this study, we identified a micropeptide produced from a putative long noncoding RNA (lncRNAs) that is important in controlling innate immunity. By studying lncRNAs in mice macrophages, we identified lncRNA 1810058I24Rik, which was downregulated in both human and murine myeloid cells exposed to LPS as well as other TLR ligands and inflammatory cytokines. Analysis of lncRNA 1810058I24Rik subcellular localization revealed that this transcript was localized in the cytosol, prompting us to evaluate its coding potential. In vitro translation with ³⁵S-labeled methionine resulted in translation of a 47 aa micropeptide. Microscopy and subcellular fractiona

SUBMITTER: Bhatta A 

PROVIDER: S-EPMC7370245 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature