Large-Scale Production of Human iPSC-Derived Macrophages for Drug Screening.

ABSTRACT: Tissue-resident macrophages are key players in inflammatory processes, and their activation and functionality are crucial in health and disease. Numerous diseases are associated with alterations in homeostasis or dysregulation of the innate immune system, including allergic reactions, autoimmune diseases, and cancer. Macrophages are a prime target for drug discovery due to their major regulatory role in health and disease. Currently, the main sources of macrophages used for therapeutic compound screening are primary cells isolated from blood or tissue or immortalized or neoplastic cell lines (e.g., THP-1). Here, we describe an improved method to employ induced pluripotent stem cells (iPSCs) for the high-yield, large-scale production of cells resembling tissue-resident macrophages. For this

SUBMITTER: Gutbier S 

PROVIDER: S-EPMC7370446 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature