Control of Early B Cell Development by the RNA N⁶-Methyladenosine Methylation.

ABSTRACT: The RNA N⁶-methyladenosine (m⁶A) methylation is installed by the METTL3-METTL14 methyltransferase complex. This modification has critical regulatory roles in various biological processes. Here, we report that deletion of Mettl14 dramatically reduces mRNA m⁶A methylation in developing B cells and severely blocks B cell development in mice. Deletion of Mettl14 impairs interleukin-7 (IL-7)-induced pro-B cell proliferation and the large-pre-B-to-small-pre-B transition and causes dramatic abnormalities in gene expression programs important for B cell development. Suppression of a group of transcripts by cytoplasmic m⁶A reader YTHDF2 is critical to the IL-7-induced pro-B cell proliferation. In contrast, the block in the large-pre-B-to-small-pre-B trans

SUBMITTER: Zheng Z 

PROVIDER: S-EPMC7371152 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature