In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases.

ABSTRACT:

Background

Immune checkpoint inhibition (ICI) alone is not efficacious for a large number of patients with melanoma brain metastases. We previously established an in situ vaccination (ISV) regimen combining radiation and immunocytokine to enhance response to ICIs. Here, we tested whether ISV inhibits the development of brain metastases in a murine melanoma model.

Methods

B78 (GD2⁺) melanoma 'primary' tumors were engrafted on the right flank of C57BL/6 mice. After 3-4 weeks, primary tumors were treated with ISV (radiation (12 Gy, day 1), α-GD2 immunocytokine (hu14.18-IL2, days 6-10)) and ICI (α-CTLA-4, days 3, 6, 9). Complete response (CR) was defined as no residual tumor observed at treatment day 90. Mice with CR were tested for immune memory by re-engraftment wit

SUBMITTER: Clark PA 

PROVIDER: S-EPMC7371368 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature