Mapping of Influenza Virus RNA-RNA Interactions Reveals a Flexible Network.

ABSTRACT: Selective assembly of influenza virus segments into virions is proposed to be mediated through intersegmental RNA-RNA interactions. Here, we developed a method called 2CIMPL that includes proximity ligation under native conditions to identify genome-wide RNA duplexes. Interactions between all eight segments were observed at multiple sites along a given segment and are concentrated at hotspots. Furthermore, synonymous nucleotide changes in a hotspot decreased the formation of RNA-RNA interactions at this site and resulted in a genome-wide rearrangement without a loss in replicative fitness. These results indicate that the viral RNA interaction network is flexible to account for nucleotide evolution. Moreover, comparative analysis of RNA-RNA interaction sites with viral nucleoprotein (NP) bi

SUBMITTER: Le Sage V 

PROVIDER: S-EPMC7372595 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature