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Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production.

ABSTRACT:

Impact statement

In order to reveal potential genotype-phenotype relationship, RT-qPCR reactions are frequently applied which require validated and reliable reference genes. With the investigation on long-term passage and fed-batch cultivation of CHO cells producing an Fc-fusion protein, four new reference genes-Akr1a1, Gpx1, Aprt, and Rps16, were identified from 20 candidates with the aid of geNorm, NormFinder, BestKeeper, and ΔCt programs and methods. This article provided more verified options in reference gene selection in related research on CHO cells.

SUBMITTER: Ma X

PROVIDER: S-EPMC7372735 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production.

Ma Xiaonan X Zhang Ling L Zhang Luming L Wang Chenglong C Guo Xiaorui X Yang Yu Y Wang Lin L Li Xiangru X Ma Ningning N

Experimental biology and medicine (Maywood, N.J.) 20200326 8

<h4>Impact statement</h4>In order to reveal potential genotype-phenotype relationship, RT-qPCR reactions are frequently applied which require validated and reliable reference genes. With the investigation on long-term passage and fed-batch cultivation of CHO cells producing an Fc-fusion protein, four new reference genes-Akr1a1, Gpx1, Aprt, and Rps16, were identified from 20 candidates with the aid of geNorm, NormFinder, BestKeeper, and ΔCt programs and methods. This article provided more verifie ...[more]

PMID: 32216463

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