Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

ABSTRACT:

Background

Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid that has a variety of physiological functions for infant development and human health. Although metabolic engineering was previously demonstrated to be a highly efficient way to rapidly increase lipid production, metabolic engineering has seldom been previously used to increase DHA accumulation in Schizochytrium spp.

Results

Here, a sensitive β-galactosidase reporter system was established to screen for strong promoters in Schizochytrium sp. Four constitutive promoters (EF-1α _p , TEF-1 _p , ccg1 _p , and ubiquitin _p ) and one methanol-induced AOX1

SUBMITTER: Han X 

PROVIDER: S-EPMC7372839 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature