Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation.

ABSTRACT: Cancer clone evolution takes place within tissue ecosystem habitats. But, how exactly tumors arise from a few malignant cells within an intact epithelium is a central, yet unanswered question. This is mainly due to the inaccessibility of this process to longitudinal imaging together with a lack of systems that model the progression of a fraction of transformed cells within a tissue. Here, we developed a new methodology based on primary mouse mammary epithelial acini, where oncogenes can be switched on in single cells within an otherwise normal epithelial cell layer. We combine this stochastic breast tumor induction model with inverted light-sheet imaging to study single-cell behavior for up to four days and analyze cell fates utilizing a newly developed image-data analysis workflow. The po

SUBMITTER: Alladin A 

PROVIDER: S-EPMC7373425 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature