Diabetes mellitus exacerbates post-myocardial infarction heart failure by reducing sarcolipin promoter methylation.

ABSTRACT:

Aims

Sarcolipin (SLN) is a key regulator of sarcoplasmic reticulum calcium-ATPase (SERCA)2a, which handles intracellular calcium re-uptake. This study was aimed to investigate the involvement of SLN in post-myocardial infarction (MI) heart failure (HF) in diabetes.

Methods and results

Diabetes/MI rat models were established. Altered SLN expression in diabetic hearts was screened out by microarray. A myocardiotropic viral vector was used to deliver siRNA to silence SLN. DNA methylation was evaluated by bisulfite sequencing. Cardiac functions were evaluated by invasive haemodynamic examinations. The SERCA2a activity, cytoplasmic calcium concentration ([Ca²⁺ ]_i ), calcium spark, and myocyte contraction were detected. Correlation between HF and diabetes was

SUBMITTER: Liu Z 

PROVIDER: S-EPMC7373908 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature